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Updated: May 17, 2026
Stimulus-specific Cortical Visual Evoked Potential Morphological Patterns
Published on: May 12, 2019
[The variation characters of EPSC-IPSC in rat visual cortex II/III pyramidal neurons during critical period of visual
Yu-yan Liu1, Xue-feng Shi, Hai-yan Mu
1Tianjin Medical University, Tianjin Eye Hospital and Institute, Tianjin, China.
Objective:
To observe the characters of EPSC-IPSC induced by paired-pulse stimulation of rat visual cortex layer II/III pyramidal neurons during critical period of visual development, and discuss their relationships, to discuss the role of short-term synaptic plasticity in the critical period of visual development of rats.
Methods:
Thirty Wistar rats were used, they were divided into P10-P12, P14-P16, P21-P23, P28-P30, P35-P37 five groups, n = 6. Whole-cell voltage clamp recording was performed, the membrane potential was clamped on -50 mV, 0 mV respectively to separate EPSC and IPSC. We set the PPR as the observation indicator, analyzed the developmental features of EPSC and IPSC induced by paired-pulse stimulation of different groups.
Results:
The PPR of layer II/III pyramidal neurons in group P10-P12, P14-P16, P21-P23, P28-P30, P35-P37 was 0.43 ± 0.08, 0.07 ± 0.08, 0.10 ± 0.10, 0.20 ± 0.07, 0.22 ± 0.12 respectively. The PPR of group P14-P16 decreased, the difference was statistically significant compared with the group before eyes open (t = -3.13, P = 0.04). The PPR of corresponding groups was 0.6036 ± 0.3021, 0.2830 ± 0.0504, 0.0287 ± 0.0907, -0.0449 ± 0.1443, -0.3089 ± 0.05553 respectively (F = 5.0799, P = 0.0037), the PPR of IPSC gradually reduced with age, and turned negative from the P28-P30 group, changed from PPF to PPD.
Conclusions:
The PPR of EPSC response to visual stimuli rapidly, but did not change significantly in the critical period of visual development (P19-P32). The short-term depression of IPSC increased gradually from the eyes open to the end of the critical period of visual development, which may play a more important role in the process of layer II/III pyramidal neurons maturation and the critical period of visual development ending.
