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Estimated copy number of Bacillus anthracis plasmids pXO1 and pXO2 using digital PCR

Timothy Straub1, Cheryl Baird, Rachel A Bartholomew

  • 1Chemical and Biological Signature Sciences Group, National Security Directorate, Pacific Northwest National Laboratory, Richland, WA 99354, United States. Timothy.Straub@pnnl.gov

Journal of Microbiological Methods
|November 13, 2012
PubMed

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Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...