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Crystallization and diffraction of an Isl1-Ldb1 complex

Morgan S Gadd1, David A Jacques, J Mitchell Guss

  • 1School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia. mgad4240@uni.sydney.edu.au

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
|November 13, 2012
PubMed

A stable intramolecular complex comprising the LIM domains of the LIM-homeodomain protein Isl1 tethered to a peptide region of Ldb1 has been engineered, purified and crystallized. The orthorhombic crystals belonged to space group P222(1), with unit-cell parameters a=57.2, b=56.7, c=179.8 Å, and diffracted to 3.10 Å resolution.

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