Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Alpha-SPECT-Mini: A Small-Animal SPECT System based on Hyperspectral Compound-Eye Gamma Cameras.

IEEE transactions on radiation and plasma medical sciences·2026
Same author

Benchtop 2D multi-pinhole x-ray fluorescence imaging system using a high energy resolution pixelated cadmium zinc telluride detector.

Medical physics·2026
Same author

Single-Step Synthesis of Cs<sub>3</sub>Bi<sub>2</sub>I<sub>9</sub> Nanocrystals for Scalable Direct X‑ray Detectors.

ACS energy letters·2025
Same author

Spatially resolved <i>operando</i> X-ray diffraction for mapping heterogeneities in Li-ion single-layer pouch cells.

Chemical communications (Cambridge, England)·2025
Same author

Neutral Ligand Triggered Low-Dimensional Reconstruction for Improving the Efficiency and Stability of Perovskite Solar Cells.

ACS applied energy materials·2024
Same author

Electronic tongue measurements as a predictor for sensory properties of vacuum-packed minced beef - A preliminary study.

Meat science·2024

Related Experiment Video

Updated: May 16, 2026

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

A laboratory system for element specific hyperspectral X-ray imaging.

Simon D M Jacques1, Christopher K Egan, Matthew D Wilson

  • 1School of Materials, The University of Manchester, Manchester, M13 9PL, UK.

The Analyst
|November 13, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a novel X-ray imaging technique that captures scattered X-rays to provide 3D structural and chemical information. This hyperspectral imaging method enhances material analysis and identification in bulk objects without sample preparation.

More Related Videos

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals
07:24

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Published on: April 14, 2020

Applying X-ray Imaging Crystal Spectroscopy for Use as a High Temperature Plasma Diagnostic
06:46

Applying X-ray Imaging Crystal Spectroscopy for Use as a High Temperature Plasma Diagnostic

Published on: August 25, 2016

Related Experiment Videos

Last Updated: May 16, 2026

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals
07:24

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Published on: April 14, 2020

Applying X-ray Imaging Crystal Spectroscopy for Use as a High Temperature Plasma Diagnostic
06:46

Applying X-ray Imaging Crystal Spectroscopy for Use as a High Temperature Plasma Diagnostic

Published on: August 25, 2016

Area of Science:

  • Physics
  • Materials Science
  • Imaging Technology

Background:

  • X-ray tomography is widely used in various fields, including medical diagnosis and industrial inspection.
  • Conventional methods only utilize transmitted X-rays, neglecting valuable information from scattered X-rays.
  • Existing techniques lack the ability to simultaneously provide detailed structural and chemical information at a voxel level.

Purpose of the Study:

  • To develop a simple, laboratory-based X-ray system for capturing scattered X-rays.
  • To demonstrate the capability of hyperspectral imaging for 3D structural and chemical analysis.
  • To showcase the potential for identifying and separating chemical species in bulk materials.

Main Methods:

  • Utilized a high-energy, laboratory-based X-ray system to capture scattered X-rays.
  • Employed hyperspectral imaging to analyze X-ray fluorescence and transmission data.
  • Applied the technique to an electronic device for component analysis.

Main Results:

  • Successfully generated 3D images with integrated structural and chemical information per voxel.
  • Demonstrated the ability to distinguish and map the atomic composition of electronic components.
  • Achieved both attenuation contrast and chemical variation imaging.

Conclusions:

  • The developed hyperspectral X-ray imaging technique offers a powerful new tool for material characterization.
  • This method enables non-destructive chemical species identification in bulk objects.
  • Potential applications span security, medical diagnostics, and materials science research.