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Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
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Published on: January 31, 2025

The Hedgehog signalling pathway regulates autophagy.

Maria Jimenez-Sanchez1, Fiona M Menzies, Yu-Yun Chang

  • 1Department of Medical Genetics, Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 0XY, UK.

Nature Communications
|November 15, 2012
PubMed
Summary

The Hedgehog signaling pathway inhibits autophagosome synthesis, a key cellular recycling process. This conserved mechanism involves specific transcription factors and may link Hedgehog signaling to autophagy regulation.

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Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
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Published on: January 31, 2025

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
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The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

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Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
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Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

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Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Developmental Biology

Background:

  • Autophagy is a fundamental cellular process for degrading damaged components and recycling cytoplasmic material, crucial for development and survival.
  • While essential, the regulation of autophagy by canonical developmental pathways like Hedgehog signaling remains poorly understood.

Purpose of the Study:

  • To investigate the regulatory relationship between the Hedgehog signaling pathway and autophagy.
  • To identify the molecular mechanisms by which Hedgehog signaling influences autophagosome synthesis.

Main Methods:

  • Utilized mammalian cell cultures and Drosophila models to study autophagy and Hedgehog pathway interactions.
  • Employed molecular biology techniques to analyze transcription factor activity (Gli2, Ci) and protein levels (PERK, eIF2α phosphorylation).

Main Results:

  • Demonstrated that the Hedgehog signaling pathway actively inhibits autophagosome synthesis under both basal and induced conditions.
  • Showed this inhibitory mechanism is conserved across species, involving Gli2 in mammals and Ci in Drosophila.
  • Identified that Hedgehog pathway activation leads to reduced PERK levels and decreased phosphorylation of eukaryotic initiation factor 2α.

Conclusions:

  • The Hedgehog signaling pathway acts as an inhibitor of autophagosome synthesis.
  • This study reveals a conserved molecular link between Hedgehog signaling and autophagy, with potential implications for developmental processes and cellular homeostasis.