Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 16, 2026

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils
08:59

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Published on: April 3, 2020

Print-to-print: a facile multi-object micro-patterning technique.

Siyuan Xing1, Siwei Zhao, Tingrui Pan

  • 1Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, Davis, CA, USA.

Biomedical Microdevices
|November 15, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal Information Fusion for Control of Rehabilitation Robots in Motor Dysfunction: A Review.

Bioengineering (Basel, Switzerland)·2026
Same author

A photoactivated nanoreactor cascade amplifies immunogenic endoplasmic reticulum stress for hepatocellular carcinoma immunotherapy.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Human PSC-derived sinoatrial node-cardiac plexus assembloids model innervation-associated maturation of pacemaker systems.

Cell stem cell·2026
Same author

Single-parameter programmed thermomechanical actuation via 3D-printed helical director fields in liquid crystal elastomers.

Nature communications·2026
Same author

Artificial Nanochannel-Mediated Ionic Transmembrane Potential for Adaptive Neuromorphic Tactile Perception.

ACS nano·2026
Same author

Deterministic Evolution of Aptamers via a Microfluidic-Integrated Robotic Platform Using Complex Exosomes as Targets.

ACS nano·2026
Same journal

A pump-free gravity-driven microfluidic chip for rapid RPA-LFS-based detection of Magnaporthe oryzae AvrPi9 gene.

Biomedical microdevices·2026
Same journal

Mechanotherapeutic biomaterials: Overcoming physical barriers to enhance intratumoral drug delivery in solid tumours.

Biomedical microdevices·2026
Same journal

Reversibly-sealable microfluidic platform for multi-molecule gradient delivery to large adherent cell cultures.

Biomedical microdevices·2026
Same journal

3D printed chip as platform to vascularize hiPSCs-derived kidney organoids.

Biomedical microdevices·2026
Same journal

Ingestible smart capsules: from engineering innovation to GI drug delivery.

Biomedical microdevices·2026
Same journal

An inexpensive, portable, refrigeration-free, ready-to-use microfluidic device for real-time multiplexed molecular detection of HIV, HBV, and HCV.

Biomedical microdevices·2026
See all related articles

A new Print-to-Print (P2P) method enables easy, out-of-cleanroom micropatterning for biological research. This versatile technique uses a standard printer and superhydrophobic films for precise cell and bacteria patterning.

Area of Science:

  • Biotechnology
  • Materials Science
  • Microfabrication

Background:

  • Micropatterning techniques are crucial for quantitative biological studies.
  • Existing methods often require cleanroom facilities, limiting accessibility.
  • There is a need for accessible, out-of-cleanroom microfabrication solutions.

Purpose of the Study:

  • To present a simple, versatile printing-based micropatterning method (Print-to-Print, P2P).
  • To enable the creation of multi-object micropatterns for biological applications.
  • To offer an accessible alternative to cleanroom-based microfabrication.

Main Methods:

  • Utilized a commercially available solid-phase printer and custom-made superhydrophobic films.
  • Employed a non-contact droplet transferring and printing process.

More Related Videos

Micro-masonry for 3D Additive Micromanufacturing
08:45

Micro-masonry for 3D Additive Micromanufacturing

Published on: August 1, 2014

Microcontact Printing of Proteins for Cell Biology
09:21

Microcontact Printing of Proteins for Cell Biology

Published on: December 5, 2008

Related Experiment Videos

Last Updated: May 16, 2026

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils
08:59

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Published on: April 3, 2020

Micro-masonry for 3D Additive Micromanufacturing
08:45

Micro-masonry for 3D Additive Micromanufacturing

Published on: August 1, 2014

Microcontact Printing of Proteins for Cell Biology
09:21

Microcontact Printing of Proteins for Cell Biology

Published on: December 5, 2008

  • No thermal or chemical treatment was required during the patterning process.

    • Main Results:

    • Successfully demonstrated a minimal feature resolution of 229 ± 17 μm.
    • Applied the P2P process for biological micropatterning on diverse substrates.
    • Achieved multi-object co-patterning on common laboratory surfaces.
    • Illustrated the reusability of superhydrophobic substrates.

    Conclusions:

    • The P2P method provides a simple, versatile, and accessible solution for micropatterning.
    • The technique is suitable for sensitive biological applications due to its non-contact nature.
    • P2P facilitates out-of-cleanroom microfabrication for broader scientific adoption.