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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells

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K Makarananda¹, G E Neal

¹Department of Pharmacology, Mahidol University, Bangkok, Thailand.

Methods in Molecular Biology (Clifton, N.J.)

|November 15, 2012

Summary

This summary is machine-generated.

Enzyme-linked immunosorbent assay (ELISA) offers sensitive detection of compounds like aflatoxins. This method is valuable for monitoring environmental toxins in food and biological samples.

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Area of Science:

Analytical Chemistry
Immunology
Environmental Science

Background:

Enzyme-linked immunosorbent assay (ELISA) is a widely applicable technique for detecting specific compounds.
The availability of specific antibodies (monoclonal or polyclonal) is crucial for ELISA's success.
ELISA is particularly useful for analyzing environmental contaminants and toxins.

Purpose of the Study:

To highlight the utility of ELISA in detecting specific compounds.
To emphasize the application of ELISA in monitoring environmental toxins.
To discuss the increasing use of ELISA for tracking carcinogenic mycotoxins, specifically aflatoxins.

Main Methods:

Utilizes specific antibodies (monoclonal or polyclonal) for compound recognition.
Employs enzyme-linked detection for sensitive and specific assays.
Applies the technique to analyze contaminated materials like foodstuffs.
Analyzes body fluids from potentially exposed individuals.

Main Results:

Demonstrates the specificity and sensitivity of ELISA for various compounds.
Confirms ELISA's effectiveness in environmental monitoring.
Shows a growing application trend in mycotoxin surveillance.

Conclusions:

ELISA is a powerful tool for sensitive and specific compound detection.
The technique is highly valuable for monitoring environmental contaminants and toxins.
ELISA plays an increasing role in assessing exposure to carcinogenic mycotoxins such as aflatoxins.