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Related Experiment Video

Updated: May 16, 2026

Optimized Minimally Invasive Transscleral Subretinal Injection Technique in Mouse
06:46

Optimized Minimally Invasive Transscleral Subretinal Injection Technique in Mouse

Published on: July 25, 2025

Optimized technique for subretinal injections in mice.

Regine Mühlfriedel1, Stylianos Michalakis, Marina Garcia Garrido

  • 1Division of Ocular Neurodegeneration, Centre for Ophthalmology, Institute for Ophthalmic Research, University of Tuebingen, Tuebingen, Germany. regine.muehlfriedel@med.uni-tuebingen.de

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
PubMed
Summary
This summary is machine-generated.

Subretinal injections are crucial for mouse gene therapy studies of inherited eye diseases. This protocol details a refined transscleral pars plana injection technique for small mouse eyes, minimizing procedural complications.

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Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
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Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

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Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
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Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Published on: August 7, 2015

Area of Science:

  • Ophthalmology
  • Gene Therapy
  • Preclinical Research

Background:

  • Subretinal injections are vital for mouse models in inherited eye disease gene therapy.
  • Small mouse eyes pose challenges for subretinal delivery, potentially impacting therapeutic outcomes.
  • Current methods may cause adverse effects due to the small ocular size.

Purpose of the Study:

  • To describe a refined transscleral pars plana subretinal injection technique for small mouse eyes.
  • To provide a reliable method for virus-mediated gene transfer in preclinical eye disease research.
  • To minimize adverse effects associated with subretinal delivery in mouse models.

Main Methods:

  • Development of a transscleral pars plana approach for subretinal injection.
  • Application in small animal eyes, specifically mouse models.
  • Focus on minimizing trauma and improving delivery precision.

Main Results:

  • Successful demonstration of a transscleral pars plana subretinal injection technique in mice.
  • The method is suitable for delivering viral vectors to photoreceptors and retinal pigment epithelium (RPE).
  • Reduced potential for adverse effects compared to other subretinal injection methods in small eyes.

Conclusions:

  • This protocol offers an improved method for subretinal injections in mouse models.
  • It facilitates accurate gene transfer for inherited eye diseases.
  • The technique is essential for advancing preclinical gene therapy research in ophthalmology.