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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
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ChIP for identification of p53 responsive DNA promoters
1Department of Biochemistry & Molecular Biology and Simon Cancer Center, Indiana University School of Medicine, Indianapolis, IN, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
Abstract:
Chromatin immunoprecipitation assay (ChIP) has been frequently used to determine whether a transcriptional regulator can bind to a specific DNA element in the chromatin content of cells. Here, we describe a detailed protocol for this assay with hands-on tips based on our own experience in working on the transcriptional regulator and tumor suppressor p53.
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