Spatial behavior and seizure-induced changes in c-fos mRNA expression in young and old rats
Monica K Chawla1, Marsha R Penner, Kathy M Olson
1ARL Division of Neural Systems, Memory and Aging and Evelyn F McKnight Brain Institute, University of Arizona, Tucson, AZ 85724-5115, USA.
Neurobiology of Aging
|November 20, 2012
Summary
Aging alters immediate-early gene expression in rat hippocampus, potentially explaining age-related memory deficits. While cell activation remains similar, changes in c-fos mRNA transcripts in aged rats may contribute to cognitive decline.
Area of Science:
- Neuroscience
- Molecular Biology
- Aging Research
Background:
- Age-related memory impairment is linked to hippocampal gene expression.
- Immediate-early genes, like c-fos, are crucial for memory consolidation.
- Neuronal activity and gene expression changes may underlie cognitive decline in aging.
Purpose of the Study:
- To investigate age-related differences in c-fos gene expression in the hippocampus.
- To determine if altered cellular activation or processing contributes to memory deficits in aged rats.
Main Methods:
- Used fluorescence in situ hybridization and RT-PCR to quantify c-fos expression.
- Examined c-fos mRNA transcripts in CA1 pyramidal and dentate gyrus granule cells of young and aged rats.
Main Results:
- No significant age differences in the number of cells expressing c-fos.
- Altered c-fos mRNA transcript levels were observed in aged rats compared to young rats.
- Cellular networks involved in activity appear maintained, but RNA transcript levels differ.
Conclusions:
- Altered c-fos mRNA transcript levels in aged rats may contribute to spatial learning and memory impairments.
- Findings suggest that changes in gene expression, not just cell numbers, are implicated in age-related cognitive deficits.
- The study highlights potential molecular mechanisms underlying cognitive aging.
