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Updated: May 16, 2026

Diagonal Method to Measure Synergy Among Any Number of Drugs
Published on: June 21, 2018
hERG drug response measured in droplet bilayers
Shiva A Portonovo1, Carl S Salazar, Jacob J Schmidt
1Department of Bioengineering, University of California, Los Angeles, 5121 Engineering V, 420 Westwood Plaza, Los Angeles, CA 90095, USA.
Abstract:
We show measurements of the human cardiac potassium ion channel Kv11.1 (hERG) in droplet bilayers incorporated directly from commercial membrane preparations of HEK293 cells. Although we do not obtain ensemble conductance kinetics and rectification observed in patch clamp measurements of hERG, ensemble currents measured in our system showed inhibition dependent on astemizole and E-4031 concentration, with IC50 values similar to those found with patch clamp. The availability of engineered HEK cells expressing a variety of ion channels, combined with the simplicity of the inhibition measurement, suggest that droplet bilayers may have considerable technological potential for determination of ion channel drug potency.
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