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Updated: May 16, 2026

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment
Published on: June 2, 2023
Fat confounds the observed apparent diffusion coefficient in patients with hepatic steatosis
Jan Hansmann1, Diego Hernando, Scott B Reeder
1Department of Clinical Radiology and Nuclear Medicine, University Medical Center Mannheim, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Purpose:
Triglyceride signal contained in peaks near the water peak remains unsuppressed by conventional fat suppression techniques used in diffusion-weighted imaging. In this work, we investigated the dependence of the apparent diffusion coefficient (ADC) on liver fat content and whether it is confounded by fat signal.
Methods:
43 patients underwent liver diffusion-weighted imaging (b = 0, 500 s/mm(2)) and single-voxel MR-spectroscopy. Proton density fat-fraction (PDFF; range 0.23-34.5%) was measured from MR-spectroscopy. A theoretical model was developed to account for the effects of fat on observed ADC, and used to correct the ADC. Linear correlation analysis was performed to assess the relationship between PDFF and ADC before and after correction.
Results:
Linear correlation analysis showed an inverse dependence between observed ADC and PDFF before correction (r(2) = 0.132; P = 0.017), and no dependence after correction (r(2) = 0.033; P = 0.24).
Conclusion:
The observed decrease in ADC in patients with fatty liver is, at least in part, artifactual due to residual fat signal near the water peak.
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