Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fibroblastic aspartoacylase suppresses TGFβ-mediated responses and cancer progression.

Nature communications·2026

Same author

Tissue-trained fibroblasts fuel invasion with lipids.

Nature metabolism·2026

Same author

Species-specific cleavage of the autophagy adaptor p62 dictates responses to TNF.

Molecular cell·2026

Same author

β-tubulin phosphorylation by Chk1 is required for normal spindle formation during cell division.

Communications biology·2026

Same author

Identification of serum protein biomarkers for pre-cancerous lesions associated with pancreatic ductal adenocarcinoma.

Molecular oncology·2026

Same author

Nucleophosmin supports WNT-driven hyperproliferation and tumor initiation.

Nature genetics·2025

Search research articles

Related Experiment Video

Updated: May 16, 2026

Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis

Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis

Published on: March 20, 2026

Quantitative mass spectrometry-based proteomics in angiogenesis.

Juan Ramon Hernandez-Fernaud¹, Steven E Reid, Lisa J Neilson

¹Vascular Proteomics Group, The Beatson Institute for Cancer Research, Glasgow, UK.

Proteomics. Clinical Applications

|November 20, 2012

Summary

Angiogenesis, the formation of new blood vessels, is vital for development and a key feature of cancer. This review explores its molecular mechanisms, models, and proteomics applications in research.

Keywords:

Angiogenesis Endothelial cell Mass spectrometry SILAC

More Related Videos

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Related Experiment Videos

Last Updated: May 16, 2026

Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis

Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis

Published on: March 20, 2026

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

Area of Science:

Vascular Biology
Oncology
Proteomics

Background:

Angiogenesis is crucial for physiological vascular development.
Aberrant angiogenesis is a hallmark of cancer, driving tumor growth.
Understanding angiogenesis requires system-wide approaches to elucidate molecular mechanisms.

Purpose of the Study:

To review the cellular and molecular dynamics of physiological angiogenesis.
To examine the deregulation of angiogenesis in cancer.
To survey models and mass spectrometry (MS) proteomics methods for studying angiogenesis.

Main Methods:

Review of current literature on angiogenesis.
Description of in vitro and in vivo models.
Overview of MS shotgun proteomics techniques.
Analysis of MS applications in endothelial cell research.

Main Results:

Detailed description of physiological vessel growth mechanisms.
Explanation of how angiogenesis is deregulated in cancer.
Survey of established and emerging research models and MS proteomics technologies.

Conclusions:

Proteomics offers powerful tools to understand endothelial cell behavior in angiogenesis.
Modern proteomics can significantly advance angiogenesis research in both health and disease.
Further integration of proteomics is essential for comprehensive insights into angiogenesis.