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A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources
Published on: January 27, 2026
Synthesis, biological evaluation, and live cell imaging of novel fluorescent duocarmycin analogs
Lutz F Tietze1, Frank Behrendt, Galina F Pestel
1Georg-August-Universität Göttingen, Institut für Organische und Biomolekulare Chemie, Tammannstrasse 2, D-37077 Göttingen, Germany. ltietze@gwdg.de
Chemistry & Biodiversity
|November 20, 2012
Abstract:
For a better understanding of the mode of action of duocarmycin and its analogs, the novel fluorescent duocarmycin derivatives 13-15 and 17b-19b were synthesized, and their bioactivity as well as their cellular uptake investigated using confocal laser scanning microscopy (CLSM) in live-cell imaging experiments.

