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Updated: May 16, 2026

Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

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Biocompatible multishell architecture for iron oxide nanoparticles.

Jana Wotschadlo¹, Tim Liebert, Joachim H Clement

¹Center of Excellence for Polysaccharide Research, Institute of Organic Chemistry and Macromolecular Chemistry (IOMC), Friedrich Schiller University of Jena, Humboldtstraße 10, 07743 Jena, Germany.

Macromolecular Bioscience

|November 20, 2012

Summary

This summary is machine-generated.

Super-paramagnetic iron oxide nanoparticles (SPIONs) were coated with multiple shells using a layer-by-layer assembly technique. These multishell nanoparticles, even with three shells, are effectively internalized by cells.

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Biofunctionalization of Magnetic Nanomaterials

Biofunctionalization of Magnetic Nanomaterials

Published on: July 16, 2020

Area of Science:

Materials Science
Nanotechnology
Biomedical Engineering

Background:

Super-paramagnetic iron oxide nanoparticles (SPIONs) are widely used in biomedical applications.
Developing methods for controlled SPIONs coating is crucial for enhancing their functionality and biocompatibility.
Multishell coatings can offer improved stability, tailored surface properties, and controlled release capabilities.

Purpose of the Study:

To demonstrate a method for creating multishell coatings on SPIONs.
To characterize the structural, magnetic, and size properties of the coated nanoparticles.
To evaluate the cellular uptake of the multishell SPIONs.

Main Methods:

Layer-by-layer assembly using carboxymethyldextran and poly(diallydimethylammonium chloride) to form polyelectrolyte complex shells.
Stepwise coating around SPION aggregates to create three distinct shells.
Characterization using Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), Magnetic Force Microscopy (MFM), and Vibrating Sample Magnetometry (VSM).
Cell culture experiments to assess cellular uptake.

Main Results:

Successful fabrication of multishell SPIONs with a stepwise increase in particle size from 96 to 327 nm.
Achieved tunable zeta potential ranging from +39 to -51 mV, indicating control over surface charge.
Microscopy and magnetic measurements confirmed the formation of core-shell structures and the integrity of the multishell architecture.
Cell culture studies demonstrated significant cellular uptake of SPIONs even with three coating layers.

Conclusions:

A robust layer-by-layer assembly method enables the creation of precisely engineered multishell SPIONs.
The resulting multishell SPIONs exhibit controlled size, surface properties, and magnetic characteristics.
The biocompatibility and cellular internalization of these multishell nanoparticles suggest their potential for various biomedical applications.