Related Experiment Video
Updated: May 16, 2026
10:35
Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
Published on: October 17, 2016
An all-fiber-optic endoscopy platform for simultaneous OCT and fluorescence imaging
Jessica Mavadia1, Jiefeng Xi, Yongping Chen
1Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA ; These authors contributed equally.
Biomedical Optics Express
|November 20, 2012
Summary
This study introduces a fiber-optic endoscope for combined optical coherence tomography (OCT) and fluorescence imaging. The dual-modality system enables simultaneous structural and molecular imaging, showing promise for clinical use.
Area of Science:
- Biomedical Optics
- Medical Imaging
- Fiber Optics
Background:
- Endoscopic imaging requires advanced techniques for simultaneous structural and molecular visualization.
- Integrating multiple imaging modalities into a single endoscopic platform presents significant technical challenges.
Purpose of the Study:
- To develop and demonstrate an all-fiber-optically based dual-modality endoscope integrating optical coherence tomography (OCT) and fluorescence imaging.
- To enable simultaneous intraluminal 3D structural and 2D surface fluorescence imaging.
Main Methods:
- Utilized a double-clad fiber (DCF) for simultaneous delivery of OCT light and fluorescence excitation, and collection of signals.
- Implemented circumferential beam scanning via a rotating reflector at the distal end.
- Integrated OCT and fluorescence modules using a custom DCF coupler and wavelength division multiplexer (WDM).
Main Results:
- Successfully demonstrated simultaneous 3D OCT and 2D fluorescence imaging in ex vivo rabbit esophagus.
- Achieved clear visualization of both structural morphologies (OCT) and fluorophore distribution (fluorescence).
- The dual-modality system operated entirely fiber-optically.
Conclusions:
- The developed dual-modality endoscope effectively combines OCT and fluorescence imaging capabilities.
- The system shows significant potential for future in vivo and clinical applications requiring simultaneous structural and molecular information.
- This fiber-optic platform offers a promising solution for advanced endoscopic diagnostics.
Related Concept Videos
01:16Confocal Fluorescence Microscopy
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
01:37Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.