Related Experiment Video
Updated: May 16, 2026

Subnanometer-Resolution Structural Determination of Hemagglutinin from Cryo-Electron Tomography of Influenza Viruses
Published on: November 7, 2025
Label-free single-particle imaging of the influenza virus by objective-type total internal reflection dark-field
Sawako Enoki1, Ryota Iino, Nobuhiro Morone
1Department of Applied Chemistry, School of Engineering, The University of Tokyo, Tokyo, Japan.
Abstract:
Here we report label-free optical imaging of single particles of the influenza virus attached on a glass surface with a simple objective-type total internal reflection dark-field microscopy (TIRDFM). The capability of TIRDFM for the imaging of single viral particles was confirmed from fine correlation of the TIRDFM images with fluorescent immunostaining image and scanning electron microscopy image. The density of scattering spots in the TIRDFM images showed a good linearity against the virus concentration, giving the limit of detection as 1.2×10(4) plaque-forming units per milliliter. Our label-free optical imaging method does require neither elaborated sample preparation nor complex optical systems, offering a good platform for rapid and sensitive counting of viral particles.
More Related Videos
Related Concept Videos
Total Internal Reflection Fluorescence Microscopy
Two-Dimensional Microscopy in Microbiology
Immunofluorescence Microscopy
The...

