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Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

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A selective amperometric sensing platform for lead based on target-induced strand release.

Feng Li¹, Limin Yang, Mingqin Chen

¹College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, China.

|November 21, 2012

Summary

A new biosensor detects lead ions (Pb(2+)) using DNA aptamers and gold nanoparticles. This method offers sensitive and selective lead detection in environmental samples.

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Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

Published on: April 18, 2013

Area of Science:

Electrochemistry
Nanomaterials Science
Biotechnology

Background:

Lead ion (Pb(2+)) contamination poses significant environmental and health risks.
Sensitive and selective detection methods for Pb(2+) are crucial for environmental monitoring.
Existing detection methods may lack sensitivity, selectivity, or require complex procedures.

Purpose of the Study:

To develop a novel amperometric biosensor for selective and sensitive detection of lead ions (Pb(2+)).
To utilize target-induced strand release mechanism for signal generation.
To demonstrate the potential of the biosensor for detecting lead in real environmental samples.

Main Methods:

Electrode modification with dendritic gold nanoparticles for enhanced surface area.
Immobilization of thiol-containing capture DNA and Pb(2+)-specific aptamers.
Detection based on methylene blue intercalation and target-induced aptamer strand release.
Amperometric measurement of electrochemical signal changes.

Main Results:

The biosensor exhibited a linear response to the logarithm of Pb(2+) concentration from 1.0 × 10(-10) M to 1.0 × 10(-7) M.
A low detection limit of 7.5 × 10(-11) M for Pb(2+) was achieved.
The biosensor demonstrated excellent selectivity for Pb(2+) over other metal ions.

Conclusions:

The developed biosensor provides a sensitive and selective platform for Pb(2+) detection.
The target-induced strand release mechanism is effective for signal transduction.
The biosensor shows promise for practical application in environmental lead monitoring.