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Isolation and characterization of hyaluronidase from scorpion (Heterometrus fulvipes) venom
M Ramanaiah1, P R Parthasarathy, B Venkaiah
1Department of Biochemistry, Sri Venkateswara University, Tirupati, India.
Biochemistry International
|January 1, 1990
Abstract:
Hyaluronidase (Hyaluronate lyase, E.C. 3.2.1.35) has been isolated from Heterometrus fulvipes scorpion venom by a combination of gel filtration on Sephadex G-75 and ion exchange chromatography on DEAE-cellulose. The enzyme preparation showed a single band on polyacrylamide gel electrophoresis and a molecular weight of 82,000. The final preparation was purified 27-fold. The optimum pH for enzyme activity was 4.0. No loss of activity was observed up to 30 degrees C and showed a sharp decrease in activity at 50 degrees C. Heparin inhibited the enzyme activity.