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Updated: May 16, 2026

Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools
Published on: November 11, 2020
Analysis of collagen fiber domain organization by Fourier second harmonic generation microscopy
Ara Ghazaryan1, Halley F Tsai, Gor Hayrapetyan
1National Taiwan University, Department of Physics, Taipei 106, Taiwan.
Abstract:
We present an automated and systematic two-dimensional discrete Fourier transform (2D-FFT) approach to analyze collagen fiber organization through the use of second harmonic generation (SHG) microscopy. Average orientations of individual domains and Ising-like order parameters introduced to characterize the correlation between orientations of adjacent domains may be used to quantitatively characterize fibrous tissues. Our approach was applied to analyze tissues including rat tail tendon, mouse skin, bovine corneas, and human corneas. We also show that collagen fiber organization in normal and keratokonus human corneas may be distinguished. The current approach may be used for the quantitative differentiation of SHG collagen fiber morphology in different tissues and may be applied for diagnostic purposes.
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