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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Enzymes02:34

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
GPI Anchoring of Proteins in the ER Membrane01:29

GPI Anchoring of Proteins in the ER Membrane

GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...

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Updated: May 16, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

The EBI enzyme portal.

Rafael Alcántara1, Joseph Onwubiko, Hong Cao

  • 1European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SD, UK. rafael.alcantara@ebi.ac.uk

Nucleic Acids Research
|November 24, 2012
PubMed
Summary
This summary is machine-generated.

A new enzyme portal integrates diverse biochemical data, simplifying access to enzyme information for researchers in fields like drug discovery and bioengineering. This resource enhances scientific discovery by consolidating protein sequences, reactions, and pathways.

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Last Updated: May 16, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
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Published on: July 12, 2022

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08:10

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Published on: August 8, 2016

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Area of Science:

  • Biochemistry and Bioinformatics
  • Enzymology
  • Bioengineering and Drug Discovery

Background:

  • Comprehensive enzyme information is crucial for interdisciplinary research.
  • Existing data is fragmented across multiple databases, hindering discovery.
  • Enzymes are vital in fields ranging from biofuels to medicine.

Purpose of the Study:

  • To develop a centralized resource for enzyme data.
  • To simplify information discovery for end-users.
  • To integrate diverse enzyme-related data classes.

Main Methods:

  • Development of a web portal at the EMBL European Bioinformatics Institute.
  • Integration of data from multiple in-house resources.
  • Focus on data classes including protein sequence, structure, reactions, pathways, and small molecules.

Main Results:

  • A unified enzyme portal is now available.
  • The portal consolidates information previously spread across separate databases.
  • User access to enzyme data is significantly streamlined.

Conclusions:

  • The enzyme portal simplifies access to critical biochemical information.
  • This integrated resource supports advancements in biochemistry, enzymology, and related fields.
  • Enhanced data accessibility facilitates interdisciplinary research and innovation.