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Related Experiment Video

Updated: May 16, 2026

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
07:29

Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment

Published on: April 22, 2019

Inflammatory events during murine squamous cell carcinoma development.

Thais Helena Gasparoto1, Carine Ervolino de Oliveira, Luisa Thomazini de Freitas

  • 1Department of Biological Sciences - Microbiology and Immunology, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. apcampan@usp.br.

Journal of Inflammation (London, England)
|November 27, 2012
PubMed
Summary

Inflammatory mediators like IL-10 and IL-17, along with elastase and activated macrophages, are imbalanced in precancerous squamous cell carcinoma (SCC), driving tumor development.

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Cell Population Analyses During Skin Carcinogenesis
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Published on: August 21, 2013

Area of Science:

  • Oncology
  • Immunology
  • Biochemistry

Background:

  • Squamous cell carcinoma (SCC) is a prevalent human cancer globally.
  • Tumorigenesis in SCC involves significant inflammatory cell infiltration and mediator production.
  • Dysregulation of these inflammatory mediators is linked to cancer progression.

Purpose of the Study:

  • To investigate the role of specific inflammatory mediators and immune cells in a multistage model of SCC.
  • To analyze the involvement of elastase (ELA), myeloperoxidase (MPO), nitric oxide (NO), and cytokines (IL-6, IL-10, IL-13, IL-17, TGF-β, TNF-α) in SCC development.
  • To assess the infiltration and activation of neutrophils and macrophages during SCC progression.

Main Methods:

  • Utilized a multistage model of SCC in an animal study.
  • Measured enzyme activities (ELA, MPO) and levels of NO, cytokines, neutrophils, and macrophages.
  • Analyzed mediator and cell levels at various time points post-carcinogen treatment (DMBA).

Main Results:

  • Increased ELA, MPO, NO, IL-10, IL-17, TNF-α, and TGF-β were observed in the precancerous microenvironment.
  • Elevated IL-6 and decreased IL-10 were noted at 4 weeks post-DMBA treatment.
  • Significant infiltration of neutrophils (GR-1+) and activated macrophages (F4/80+/GR-1+) correlated with tumor lesions and inflammatory mediators.

Conclusions:

  • An imbalance of inflammatory mediators, driven by neutrophils and macrophages, contributes to pro-tumorigenic alterations in precancerous SCC.
  • These findings highlight the complex interplay between inflammation and SCC development.