Related Experiment Video

Updated: May 16, 2026

Intra-Peritoneal Transplantation for Generating Acute Myeloid Leukemia in Mice
10:02

Intra-Peritoneal Transplantation for Generating Acute Myeloid Leukemia in Mice

Published on: January 6, 2023

Overexpression of amyloid precursor protein in acute myeloid leukemia enhances extramedullary infiltration by MMP-2

Ling Jiang1, Guopan Yu, Wei Meng

  • 1Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|November 27, 2012
PubMed

Insights

Amyloid precursor protein (APP) overexpression in acute myeloid leukemia (AML) correlates with extramedullary infiltration and poorer survival. Reducing APP inhibits leukemia cell migration and MMP-2 expression, suggesting a role in disease spread.

Area of Science:

  • Hematology
  • Molecular Biology
  • Oncology

Background:

  • Extramedullary infiltration (EMI) in leukemia patients is linked to a worse prognosis.
  • Amyloid precursor protein (APP) is implicated in cell adhesion, motility, and proliferation.
  • The prognostic role of APP in acute myeloid leukemia (AML) remains uninvestigated.

Purpose of the Study:

  • To investigate the expression and prognostic significance of APP in AML.
  • To explore the role of APP in the extramedullary infiltration of leukemia.
  • To elucidate the mechanism by which APP influences leukemia cell behavior.

Main Methods:

  • Analysis of APP expression in AML/ETO(+) leukemia patients.
  • In vitro knockdown of APP using small interfering RNA (siRNA) in Kasumi-1 cells.
  • Assessment of cell migration and proliferation using Transwell assays.
  • Quantitative real-time polymerase chain reaction and Western blot to evaluate MMP-2 expression.

Main Results:

  • AML/ETO(+) patients overexpressing APP showed a higher incidence of EMI and reduced long-term survival.
  • siRNA-mediated knockdown of APP significantly impaired leukemia cell migration but not proliferation in vitro.
  • APP knockdown led to decreased expression of MMP-2 in Kasumi-1 cells.

Conclusions:

  • APP overexpression is a negative prognostic factor in AML, associated with increased extramedullary infiltration.
  • APP plays a crucial role in regulating leukemia cell migration.
  • APP may mediate extramedullary infiltration in leukemia through the MMP-2 pathway.

More Related Videos

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia06:33

Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia

Published on: November 10, 2023

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...