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Characteristic immunophenotyping artefact seen in patients with anti-mouse immunoglobulin antibodies

D Wilks1, N Byrom, L Walker

  • 1Retroviral Research Group, Clinical Research Centre, Harrow.

Cytometry
|January 1, 1990
PubMed

Patients treated with murine monoclonal antibodies develop anti-mouse immunoglobulin antibodies which can interfere with flow cytometry of lymphocytes, giving rise to high levels of nonspecific staining. This artefact can be avoided by using separated, washed lymphocytes for immunophenotyping.

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