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Updated: May 16, 2026

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
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Published on: July 4, 2007

Dengue virus: isolation, propagation, quantification, and storage.

Freddy Medina1, Juan Francisco Medina, Candimar Colón

  • 1Centers for Disease Control and Prevention, Division of Vector Borne Infectious Diseases, Dengue Branch, San Juan, Puerto Rico.

Current Protocols in Microbiology
|November 28, 2012
PubMed
Summary

Dengue virus (DENV) isolation remains crucial for research and diagnostics, despite newer methods. This study details established DENV isolation, serotyping, and quantification techniques for laboratory use.

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Last Updated: May 16, 2026

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
15:25

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination

Published on: July 4, 2007

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Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes
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Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes

Published on: August 31, 2022

Area of Science:

  • Virology
  • Infectious Diseases
  • Public Health

Background:

  • Dengue is a significant global health concern caused by four serotypes of the dengue virus (DENV).
  • Transmission occurs through Aedes mosquito vectors, with disease severity ranging from mild fever to life-threatening conditions.
  • While modern diagnostics exist, DENV isolation is the gold standard for characterization and research.

Purpose of the Study:

  • To present standardized experimental methods for DENV isolation, serotyping, propagation, and quantification.
  • To provide a reference for researchers and diagnostic laboratories working with DENV.
  • To highlight the continued importance of virus isolation in DENV research.

Main Methods:

  • Isolation of DENV from clinical samples using mammalian and mosquito cell cultures.
  • Isolation via inoculation of Aedes mosquitoes.
  • Detailed procedures for serotyping, propagation, and quantification of DENV.

Main Results:

  • Established protocols for successful DENV isolation and characterization.
  • Demonstrated the utility of virus isolation for genetic sequencing and experimental studies.
  • Provided a comprehensive guide to common DENV laboratory procedures.

Conclusions:

  • DENV isolation is indispensable for detailed virus characterization, serotyping, and research.
  • Standardized isolation methods ensure reliable data for understanding DENV.
  • These techniques are vital for reference laboratories and ongoing dengue research efforts.