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Updated: Jan 7, 2026

Amplicon Sequencing using the Long-Read Sequencing Technologies
Published on: August 29, 2025
Identification of lineages and sublineages of the Mycobacterium tuberculosis complex using advanced molecular
María Aznar1, Mariana G López2, María José Torres3
1Servicio de Microbiología y Parasitología clínica, UCEIMP, Hospital Universitario Virgen del Rocío. Instituto de Biomedicina de Sevilla (IBiS), Grupo de Investigación de Microbiología clínica y Molecular, Línea Micobacterias, Seville, Spain.
Introduction:
Tuberculosis (TB) in the Virgen del Rocío university hospital health area, included in the Seville Health District, Spain, exceeds the rate of 17 cases/100,000 inhabitants. Within the Mycobacterium tuberculosis complex (MTBC), M. tuberculosis sensu stricto comprises seven phylogenetic lineages (L1-L4 and L7-L9). The objective of this work is to know the circulating lineages in the area.
Methods:
Molecular typing and sequencing were performed to identify circulating lineages from 2015 to 2022.
Results:
From 2015 to 2019, the following were identified: L4 (95.95%, n = 355), L2 (1.62%, n = 6), and L6 (1.08%, n = 4). From 2020 to 2022: L4 94.62% (n = 123); L1 1.5% (n = 2); L3 1.5% (n = 2) and L2 0.76% (n = 1).
Conclusions:
The emergence of different lineages in our area and their recognition are key to contributing to disease control. These findings demonstrate the need to continue surveillance and apply sequencing to understand and control the dynamics of TB transmission.
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