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Updated: May 16, 2026

Assessment of Labile Organic Carbon in Soil Using Sequential Fumigation Incubation Procedures
Published on: October 29, 2016
[Effects of chlorimuron-ethyl and urea on soil microbial biomass carbon and nitrogen and soil inorganic nitrogen]
Huan-Bo Tan1, Xin-Yu Li, Hui-Wen Zhang
1State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, China. tan.huanbo@gmail.com
Abstract:
A microcosm experiment was conducted to study the effects of different concentration chlorimuron-ethyl (20, 200, and 2000 microg x kg(-1) soil) and its combination with urea (120 mg x kg(-1) soil) on the dynamic changes of soil microbial biomass carbon and nitrogen and soil nitrate nitrogen and ammonium nitrogen. Applying chlorimuron-ethyl alone decreased the soil microbial biomass carbon and nitrogen throughout the experiment period (60 days), and the decrement increased with increasing chlorimuron-ethyl concentration. Chlorimuron-ethyl had little effects on the soil ammonium nitrogen and nitrite nitrogen in the early period of the experiment, but increased the soil ammonium nitrogen in the mid-period (15 d) and the soil nitrate nitrogen in the late period (after 30 days) significantly. Both urea addition and its combination with chlorimuron-ethyl increased the soil microbial biomass carbon and nitrogen obviously in a short time, but the effect of combined addition of urea and chlorimuron-ethyl weakened then. Applying urea and its combination with chlorimuron-ethyl resulted in a lasting increase of soil nitrate nitrogen and ammonium nitrogen.
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