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Updated: May 16, 2026
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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
Published on: March 24, 2023
Performance of a novel BD stem cell enumeration kit on two flow cytometry systems
I Omana-Zapata1, F Oreizy, F Mosqueda
1BD Biosciences, San Jose, CA 95131, USA. Imelda_Omana-Zapata@bd.com
International Journal of Laboratory Hematology
|November 30, 2012
Summary
The BD™ Stem Cell Enumeration (SCE) Kit provides precise and reproducible quantification of viable CD34⁺ cells using flow cytometry. This validated kit ensures reliable stem cell counts for clinical applications like hematopoietic stem cell transplantation.
Area of Science:
- Flow cytometry
- Hematopoietic stem cell enumeration
Background:
- Accurate stem cell enumeration is crucial for hematopoietic stem cell transplantation.
- Flow cytometry is a standard method for assessing CD34⁺ cells.
Purpose of the Study:
- Evaluate the precision, linearity, and stability of the BD™ Stem Cell Enumeration (SCE) Kit.
- Assess performance on two distinct flow cytometry platforms.
Main Methods:
- Utilized a modified ISHAGE gating strategy with a viability dye.
- Tested precision with high/low controls and linearity with cell dilutions.
- Assessed stability using mobilized leukapheresis specimens.
Main Results:
- Demonstrated high precision with minimal variability in CD34⁺ cell counts.
- Established a linearity range of 0-1000 cells/μL (R² = 0.99).
- Confirmed sample stability up to 24 hours post-collection and 60 minutes post-stain/lyse.
Conclusions:
- The BD SCE Kit offers consistent and reproducible results on tested flow cytometry systems.
- The kit is suitable for reliable CD34⁺ cell enumeration in clinical settings.
