Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
The Skin Microbiota01:27

The Skin Microbiota

The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: A novel extracellular role for tissue transglutaminase in matrix-bound VGF-mediated angiogenesis.

Cell death & disease·2026
Same author

Editorial Expression of Concern: The adenine nucleotide translocator 1 acts as a type 2 transglutaminase substrate: implications for mitochondrial-dependent apoptosis.

Cell death and differentiation·2026
Same author

Correction: p73 and caspase-cleaved p73 fragments localize to mitochondria and augment TRAIL-induced apoptosis.

Oncogene·2025
Same author

BRD4 sustains p63 transcriptional program in keratinocytes.

Biology direct·2024
Same author

Involvement of transcribed lncRNA uc.291 and SWI/SNF complex in cutaneous squamous cell carcinoma.

Discover oncology·2022
Same author

Correction to: p63 regulates the caspase-8-FLIP apoptotic pathway in epidermis.

Cell death and differentiation·2021
Same journal

Mitochondrial-endoplasmic reticulum interactions in lung diseases.

Cell death & disease·2026
Same journal

A ZDHHC3-USP5-PTRF axis links palmitoylation to ferroptosis-associated phenotypes in melanoma.

Cell death & disease·2026
Same journal

Small molecule-mediated activation of Notch signal transduction.

Cell death & disease·2026
Same journal

OT-55 reshapes tolerogenic BH3-mimetic-induced apoptosis toward immunogenic cell death in acute myeloid leukemia, potentiating PD-1/Tim-3 blockade.

Cell death & disease·2026
Same journal

FOXQ1 activates GLT8D2 to enhance CCL2 N-glycosylation and promote prostate cancer bone metastasis.

Cell death & disease·2026
Same journal

PCGF1-mediated bivalent promoter remodeling enables NK cell immune evasion in non-small cell lung cancer.

Cell death & disease·2026
See all related articles

Related Experiment Video

Updated: May 16, 2026

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
09:14

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Published on: August 22, 2016

MicroRNA-203 contributes to skin re-epithelialization.

G Viticchiè1, A M Lena, F Cianfarani

  • 1Department of Experimental Medicine and Surgery, University of Tor Vergata, Via Montpellier, 1, Rome 00133, Italy.

Cell Death & Disease
|November 30, 2012
PubMed
Summary
This summary is machine-generated.

MicroRNA-203 (miR-203) downregulation promotes keratinocyte migration and proliferation, crucial for wound healing. This study identifies miR-203's role in regulating epidermal homeostasis after skin injury.

More Related Videos

Demonstration of the Rat Ischemic Skin Wound Model
08:35

Demonstration of the Rat Ischemic Skin Wound Model

Published on: April 1, 2015

Related Experiment Videos

Last Updated: May 16, 2026

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
09:14

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Published on: August 22, 2016

Demonstration of the Rat Ischemic Skin Wound Model
08:35

Demonstration of the Rat Ischemic Skin Wound Model

Published on: April 1, 2015

Area of Science:

  • Molecular Biology
  • Dermatology
  • Wound Healing Research

Background:

  • Keratinocyte proliferation and migration are essential for epidermal wound healing.
  • The precise molecular mechanisms governing these cellular responses are not fully understood.

Purpose of the Study:

  • To investigate the role of microRNA-203 (miR-203) in mouse skin wound healing.
  • To elucidate the molecular targets and regulatory functions of miR-203 during epidermal regeneration.

Main Methods:

  • In situ hybridization to analyze miR-203 expression in wounded mouse epidermis.
  • Subcutaneous injection of antagomiR-203 in newborn mice to inhibit miR-203 activity in vivo.
  • Identification of miR-203 target mRNAs (RAN and RAPH1).

Main Results:

  • miR-203 expression was downregulated in proliferating keratinocytes at the wound edge ('migrating tongue').
  • miR-203 was highly expressed in differentiating keratinocytes outside the wound area.
  • Inhibition of miR-203 in vivo enhanced the inverse correlation with target mRNAs RAN and RAPH1.
  • These targets are implicated in keratinocyte proliferation and migration.

Conclusions:

  • miR-203 plays a critical role in regulating keratinocyte proliferation and migration during wound re-epithelialization.
  • miR-203 contributes to the re-establishment of epidermal homeostasis in injured skin.
  • The findings highlight miR-203 as a potential therapeutic target for enhancing wound healing.