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Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

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Related Experiment Video

Updated: May 16, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Automated high-speed CE system for multiple samples.

Qi Li1, Ting Zhang, Ying Zhu

  • 1Department of Chemistry, Institute of Microanalytical Systems, Zhejiang University, Hangzhou, PR China.

Electrophoresis
|November 30, 2012
PubMed
Summary
This summary is machine-generated.

A novel high-speed capillary electrophoresis (CE) system automates sample introduction for rapid analysis. This system achieves fast and efficient separation of multiple samples, enabling high throughput for biochemical analyses.

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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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Last Updated: May 16, 2026

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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
10:05

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Separation Science

Background:

  • Traditional capillary electrophoresis (CE) systems can be time-consuming for analyzing multiple samples.
  • Automated sample handling is crucial for increasing throughput in analytical methods.

Purpose of the Study:

  • To develop a high-speed CE system with automated sample introduction for rapid, multi-sample analysis.
  • To optimize sample injection for high separation speed and efficiency.

Main Methods:

  • Development of a CE system utilizing a short capillary and an automated sample introduction device with a multi-well plate and x-y-z translation stage.
  • Implementation of a spontaneous injection method for picoliter-scale sample loading.
  • Optimization of conditions to achieve a 40 μm sample plug in a 50 μm inner diameter capillary.
  • Utilized Laser-Induced Fluorescence (LIF) detection for analyzing FITC-labeled amino acids.

Main Results:

  • Achieved separation of five FITC-labeled amino acids (arginine, phenylalanine, glycine, glutamic acid, asparagine) within 15 seconds over a 1.5 cm effective separation length.
  • Demonstrated high separation efficiency, with plate heights ranging from 0.89 to 1.26 μm.
  • Reported good repeatability for peak heights (2.4-2.7%, n=20) using an inner standard.
  • Successfully performed consecutive separations of 20 different FITC-labeled amino acid samples in under 6 minutes.

Conclusions:

  • The developed high-speed CE system enables rapid and efficient separation of multiple samples.
  • Automated picoliter-scale sample injection significantly enhances analytical throughput.
  • The system demonstrates excellent performance for analyzing biomolecules like labeled amino acids.