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Interference by pralidoxime (PAM) salts in clinical laboratory tests
Sumika Nagase1, Katsunori Kohguchi, Kaoru Tohyama
1Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|December 1, 2012
Summary
Pralidoxime (PAM) iodide and potassium iodide can falsely elevate glucose readings on certain meters. PAM salts also interfere with 8 other clinical lab tests measured by spectrophotometry.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Toxicology
Background:
- Medications can interfere with clinical laboratory test results.
- Pralidoxime (PAM) salts are used to treat organophosphorus poisoning.
- The impact of PAM salts on laboratory tests was previously unexamined.
Purpose of the Study:
- To investigate the effects of pralidoxime (PAM) salts on clinical laboratory test results.
- To determine if PAM salts interfere with glucose measurements.
- To assess the impact of PAM salts on other common clinical assays.
Main Methods:
- Examined PAM salt effects on glucose (GLU) using point-of-care testing (POCT) and self-monitoring of blood glucose (SMBG) meters.
- Evaluated PAM salt interference with glucose using biochemical autoanalyzers.
- Assessed PAM salt effects on 14 other clinical laboratory tests using absorption spectrophotometry.
Main Results:
- PAM iodide and potassium iodide increased GLU values on enzyme electrode-based POCT and SMBG meters.
- PAM iodide and PAM chloride altered results for 8 of 14 tests analyzed by absorption spectrophotometry at 340 nm.
- PAM interacted with alkaline buffer reagents, causing a shift in its absorption spectrum.
Conclusions:
- PAM iodide can lead to falsely high glucose readings with enzyme electrode methods.
- PAM salts interfere with multiple clinical laboratory tests analyzed via absorption spectrophotometry.
- Understanding these interferences is crucial for accurate patient diagnosis and treatment.
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