Microbubble sonodestruction rate as a metric to evaluate sonoporation efficiency
Mindaugas Tamosiūnas1, Rytis Jurkonis, Lluis M Mir
1Department of Biology, Vytautas Magnus University, Vileikos 8, LT-44404 Kaunas, Lithuania.
Summary
The microbubble sonodestruction rate can predict sonoporation efficiency. This finding enables a new dosimetric method for evaluating ultrasound-mediated drug delivery, crucial for cancer therapy.
Area of Science:
- Biomedical Engineering
- Acoustic Cavitation
- Drug Delivery
Background:
- Sonoporation efficiency relies on microbubble cavitation and destruction rates.
- Developing accurate dosimetry for sonoporation is essential for therapeutic applications.
Purpose of the Study:
- To investigate microbubble sonodestruction rate as a parameter for sonoporation dosimetry.
- To evaluate the correlation between microbubble sonodestruction and sonoporation efficiency.
Main Methods:
- Utilized 12-MHz diagnostic ultrasound in B-scan mode to assess microbubble sonodestruction rate.
- Exposed Chinese hamster ovary cells to 880 kHz therapeutic ultrasound with microbubbles.
- Quantified sonoporation efficiency via bleomycin sonotransfer.
Main Results:
- Low sonodestruction rates (<0.5 s⁻¹) showed minimal cell viability impact and <10% sonoporation.
- Higher sonodestruction rates led to a linear increase in sonoporation efficiency.
- Sonoporation efficiency plateaued at 5 s⁻¹, indicating optimal ultrasound parameters.
Conclusions:
- The microbubble sonodestruction rate serves as a reliable predictor for sonoporation outcomes.
- This study establishes a foundation for an implicit dosimetric method for sonoporation.
- Findings support optimizing ultrasound parameters for enhanced drug delivery via sonoporation.
