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Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection
Published on: June 16, 2020
Recognition and enrichment specificity of Fe₃O₄ magnetic nanoparticles surface modified by chitosan and
Xiuheng Xue1, Juhua Wang, Lin Mei
1College of Tea & Food Science and Technology, Anhui Agricultural University, Key Laboratory of Analysis and Detection Technology for Food Safety, Hefei, Anhui 230036, China.
Abstract:
Fe(3)O(4) modified by chitosan (CTS) and Staphylococcus aureus enterotoxin A (SEA) antiserum immunomagnetic beads (Fe(3)O(4)-CTS-SEA-IB) that targeted enrich SEA were prepared in aqueous solution and using CTS and SEA antiserum as surface modification stabilizer. Recognition experiments of the prepared Fe(3)O(4)-CTS-SEA-IB on SEA were conducted to determine adsorption capacity and recognition specificity. The results showed that the SEA antiserum were prepared and successfully conjugated onto Fe(3)O(4)-modificated CTS magnetic beads. The Fe(3)O(4)-CTS-SEA immunomagnetic beads displayed a high adsorption capacity and recognition specificity for SEA, and the adsorption quantity could reach 6.48 × 10(-3) μmol/g. The specificity evaluation results showed that the Fe(3)O(4)-CTS-SEA immunomagnetic beads had high enrichment and affinity property for SEA compared to SEB (Staphylococcus aureus enterotoxin B) and SPA (Staphylococcus aureus protein A).
