Related Experiment Video
Updated: May 16, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
Published on: July 5, 2016
Aberration compensation for objective phase curvature in phase holographic microscopy
Kyung Won Seo1, Yong Seok Choi, Eun Seok Seo
1Center for Biofluid and Biomimic Research, Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, South Korea.
Abstract:
In this study, we proposed a simple and fast numerical approach to compensate for aberrations induced by objective phase curvature. This method is based on the extraction of virtual background phase from reconstructed phase values using a line profile, followed by subtraction of the virtual background phase from the reconstructed phase image. The performance and feasibility of the method were demonstrated by applying it to the phase imaging of polystyrene microspheres and red blood cells.
Related Concept Videos
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Focusing of Light in the Eye
Influence of Earth's Curvature and Atmospheric Refraction on Leveling

