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Optimization of lipase production by Burkholderia sp. using response surface methodology
Chia-Feng Lo1, Chi-Yang Yu, I-Ching Kuan
1Department of Bioengineering, Tatung University, No 40, Sec. 3, Zhongshan N. Rd., Taipei 104, Taiwan. slee@ttu.edu.tw
Abstract:
Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a faced-centered central composite design (FCCCD). The analysis of variance revealed that the established model was significant (p < 0.01). The optimized medium containing 0.65% olive oil (v/v), 2.42% tryptone (w/v) and 0.15% Tween-80 (v/v) resulted in a maximum activity of 122.3 U/mL, about three fold higher than that in basal medium. Approximately 99% of validity of the predicted value was achieved.
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