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Label-free DNA detection using the narrow side of funnel-type etched nanopores

Yohai Mandabi1, Dietmar Fink, Lital Alfonta

  • 1Avram and Stella Goldstein-Goren Department of Biotechnology Engineering and the Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 84105, Israel.

Biosensors & Bioelectronics
|December 5, 2012
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...