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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Engineering molecular beacons for intracellular imaging.

Cuichen Sam Wu1, Lu Peng, Mingxu You

  • 1Center for Research at Bio/Nano Interface and Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA.

International Journal of Molecular Imaging
|December 5, 2012
PubMed
Summary
This summary is machine-generated.

Novel molecular beacons (MBs) offer enhanced fluorescence and stability for real-time DNA/RNA imaging in cells. These engineered probes overcome limitations of conventional MBs, enabling precise single-cell analysis in biotechnology and medicine.

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Area of Science:

  • Molecular biology
  • Biotechnology
  • Chemical analysis

Background:

  • Molecular beacons (MBs) are DNA/RNA probes with hairpin structures for targeted detection.
  • Their
  • Purpose of the Study
  • Main Methods
  • Main Results
  • Conclusions

Purpose of the Study:

  • To engineer novel molecular beacons (MBs) with improved fluorescence and biostability.
  • To enhance resistance to nuclease degradation using unnatural nucleotide bases.
  • To enable real-time single-cell imaging of DNA/RNA.

Main Methods:

  • Engineering novel DNA hairpin structures for MBs.
  • Incorporation of unnatural nucleotide bases to enhance nuclease resistance.
  • Optimization of fluorescence signal and reduction of background fluorescence.

Main Results:

  • Achieved improved fluorescence signal and reduced background noise.
  • Demonstrated increased resistance of MBs to nuclease degradation.
  • Successfully applied engineered MBs for single-cell imaging.

Conclusions:

  • Engineered MBs offer superior performance over conventional probes.
  • Enhanced MBs are suitable for sensitive and stable real-time cellular imaging.
  • These advancements hold promise for chemical analysis, biotechnology, and clinical diagnostics.