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Updated: May 16, 2026

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
Published on: April 22, 2022
Polyphenols excreted in urine as biomarkers of total polyphenol intake
Alexander Medina-Remón1, Anna Tresserra-Rimbau, Sara Arranz
1Nutrition & Food Science Department, XaRTA, Instituto de Investigaciónen Nutrición y Seguridad Alimentaria, Pharmacy School, University of Barcelona, Barcelona, Spain.
Background:
Nutritional biomarkers have several advantages in acquiring data for epidemiological and clinical studies over traditional dietary assessment tools, such as food frequency questionnaires. While food frequency questionnaires constitute a subjective methodology, biomarkers can provide a less biased and more accurate measure of specific nutritional intake. A precise estimation of polyphenol consumption requires blood or urine sample biomarkers, although their association is usually highly complex.
Results:
This article reviews recent research on urinary polyphenols as potential biomarkers of polyphenol intake, focusing on clinical and epidemiological studies. We also report a potentially useful methodology to assess total polyphenols in urine samples, which allows a rapid, simultaneous determination of total phenols in a large number of samples.
Conclusion:
This methodology can be applied in studies evaluating the utility of urinary polyphenols as markers of polyphenol intake, bioavailability and accumulation in the body.
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