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Related Experiment Video

Updated: May 16, 2026

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
17:39

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

Published on: July 16, 2017

Method development for fecal lipidomics profiling.

Katherine E Gregory1, Susan S Bird, Vera S Gross

  • 1Boston College, W.F. Connell School of Nursing, 140 Commonwealth Avenue, Chestnut Hill, Massachusetts 02467, USA.

Analytical Chemistry
|December 6, 2012
PubMed
Summary
This summary is machine-generated.

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Researchers developed a new lipidomics method for fecal analysis, improving gut health insights, especially for premature infants. This approach enhances understanding of disease and nutrition in vulnerable populations.

Area of Science:

  • Gastroenterology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Understanding gut physiology and disease requires robust fecal analysis methods.
  • Lipidomics offers a promising approach for analyzing gut health and disease.
  • High-risk populations, like premature infants, need improved diagnostic tools.

Purpose of the Study:

  • To develop a simple, sensitive, and quantitatively precise method for profiling intact lipids in fecal material.
  • To establish a new paradigm for assessing nutritional health, inflammation, and infectious disease in vulnerable populations.

Main Methods:

  • Utilized two complementary extraction chemistries: dichloromethane (DCM) and methyl tert-butyl ether/hexafluoroisopropanol (MTBE) mixture.
  • Employed liquid chromatography-high-resolution mass spectrometry with all ion higher energy collisional dissociation fragmentation.

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Related Experiment Videos

Last Updated: May 16, 2026

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples
17:39

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

Published on: July 16, 2017

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

  • Assessed lipid profiles in positive and negative ionization modes, with and without pressure cycling.

    • Main Results:

    • Successfully profiled over 500 intact lipophilic species, identifying more than 300 lipids across 6 LIPID MAPS categories.
    • Demonstrated differential lipid extraction based on hydrophobicity using DCM and MTBE solvents.
    • Showcased pressure cycling's ability to differentially increase lipid yield.

    Conclusions:

    • The developed lipidomics platform provides a novel and comprehensive approach to fecal lipid analysis.
    • This method enhances lipid characterization through class-specific and lipid-specific fragments.
    • Represents a significant advancement for assessing health and disease in vulnerable infant populations.