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Mating type gene (MAT1-1) in Japanese isolates of Trichophyton rubrum

Rui Kano1, Maiko Isizuka, Masataro Hiruma

  • 1Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866, Fujisawa, Kanagawa, 252-0880, Japan. kano@brs.nihon-u.ac.jp

Mycopathologia
|December 6, 2012
PubMed

Insights

This study investigated Trichophyton rubrum, a common cause of fungal infections. PCR analysis revealed all Japanese and canine isolates possess the MAT1-1 gene, suggesting a uniform mating type.

Area of Science:

  • Medical Mycology
  • Molecular Biology
  • Epidemiology

Background:

  • Trichophyton rubrum is the leading cause of human dermatophytosis globally.
  • The sexual cycle (teleomorph) of T. rubrum remains unidentified.
  • Understanding mating type distribution is crucial for its epidemiology and ecology.

Purpose of the Study:

  • To confirm the presence of a mating type locus in Japanese T. rubrum isolates.
  • To correlate mating type (MAT) genotype with mating type.
  • To investigate the epidemiological and ecological characteristics of T. rubrum.

Main Methods:

  • Polymerase Chain Reaction (PCR) analysis was employed.
  • Mating type sequences were analyzed.
  • Clinical isolates from 206 human tinea pedis and tinea unguium cases in Japan and 10 canine dermatophytosis cases were examined.

Main Results:

  • PCR detected the MAT1-1 gene in all examined human and animal isolates of T. rubrum.
  • No MAT1-2 gene was detected.
  • All isolates were predicted to exhibit the (-) mating type.

Conclusions:

  • Japanese T. rubrum isolates, including those from human and canine infections, predominantly possess the MAT1-1 gene.
  • This suggests a potential lack of sexual recombination or a skewed mating type distribution in the studied populations.
  • Further research is needed to understand the implications for T. rubrum's lifecycle and antifungal resistance.

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