Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conservation and variation in Orthopoxvirus genome structure.

M Mackett, L C Archard

    The Journal of General Virology
    |December 1, 1979
    PubMed
    Summary

    Orthopoxvirus genomes share conserved internal DNA sequences, but exhibit type-specific variations, primarily at their termini. These findings aid in understanding Orthopoxvirus diversity and evolution.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The live vector approach-viruses.

    World journal of microbiology & biotechnology·2014
    Same author

    Manipulation of vaccinia virus vectors.

    Methods in molecular biology (Clifton, N.J.)·2011
    Same author

    Herpes simplex virus type 1 infection in rheumatic valvar disease.

    Heart (British Cardiac Society)·2004
    Same author

    Enterovirus related metabolic myopathy: a postviral fatigue syndrome.

    Journal of neurology, neurosurgery, and psychiatry·2003
    Same author

    Enterovirus replication in valvular tissue from patients with chronic rheumatic heart disease.

    European heart journal·2002
    Same author

    A vector with transcriptional terminators increases efficiency of cloning of an RNA virus by reverse transcription long polymerase chain reaction.

    Journal of molecular microbiology and biotechnology·2002

    Area of Science:

    • Molecular Biology
    • Virology
    • Genomics

    Background:

    • Orthopoxviruses are a genus of double-stranded DNA viruses that cause significant diseases in mammals.
    • Understanding the genomic structure of Orthopoxviruses is crucial for distinguishing between different strains and for developing diagnostic and therapeutic strategies.

    Purpose of the Study:

    • To analyze and compare the genomic structures of representative Orthopoxvirus strains using restriction endonuclease digestion.
    • To identify conserved and variable regions within Orthopoxvirus genomes and characterize terminal sequences.

    Main Methods:

    • DNA from Orthopoxvirus strains (rabbitpox, vaccinia, monkeypox, variola, cowpox, ectromelia) was cleaved with restriction enzymes HindIII, XhoI, and SmaI.
    • Genome molecular weights were estimated.
    • Physical maps of cleavage sites were generated, and terminal restriction fragments were analyzed for cross-hybridization.

    Main Results:

    • Genome molecular weights ranged from approximately 120 x 10^6 (rabbitpox) to 145 x 10^6 (cowpox).
    • Physical maps showed conserved internal regions (approx. 30 x 10^6 mol. wt. for HindIII sites) with type-specific differences, particularly in ectromelia virus.
    • Near-terminal covalent cross-links and homologous sequences (up to 6 x 10^6 mol. wt.) were identified in terminal fragments, with variola strains showing a unique deficiency at one terminus.

    Conclusions:

    • Orthopoxvirus genomes display significant conservation in internal DNA sequences, with variations primarily occurring in near-terminal regions.
    • Restriction endonuclease analysis effectively distinguishes between Orthopoxvirus types and reveals genomic structural similarities and differences.
    • The presence of terminal cross-links and homologous sequences suggests mechanisms for genome stability and recombination within the Orthopoxvirus genus.

    Related Experiment Videos