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Updated: May 16, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
[Determination of nine kinds of polycyclic aromatic hydrocarbons in serum by high performance liquid chromatography]
Changming Ding1, Lei Zheng, Shaobin Lin
1Institute for Environment Hygiene and Health Related Product Safety, Chinese CDC, Beijing 100021, China.
Objective:
To develop a high performance liquid chromatography (LC) method for simultaneously determination of polycyclic aromatic hydrocarbons (PAHs) in serum.
Methods:
PAHs could be separated completely by using C18 column and detected by fluorescence detector at wavelengths (Ex = 340 nm, Em = 425 nm). The mobile phase A was acetonitrile and mobile phase B was water with a flow rate at 1.0 ml/min.
Results:
The concentration of PAHs achieved a good linear relation with the peak area. The correlation coefficient was in the range of 0.9941-0.9999. The recoveries were in the range of 91.4%-99.8% and the RSD were in the range of 2.56%-12.7%. The detection limits were in the range of 0.05-0.10 ng/ml.
Conclusion:
The method was simple, rapid, sensitive and accurate, and suitable for the detection of PAHs in serum.
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