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[Study on HPLC fingerprints of Chrysanthemi Flos before and after sulfur-fumigation]
Xiao Liu1, Hao Cai, Xiao-qing Ma
1College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China. liuxiao04_0@163.com
Objective:
To evaluate the quality of Chrysanthemi Flos before and after sulfur-fumigation by analysis of HPLC fingerprints.
Methods:
RP-HPLC method was applied to establish the chromatographic fingerprint. The separation was performed on a Waters C, (250 mm x 4.6 mm, 5 microm) column with a gradient elution composed of acetonitrile-aqueous solution containing 0.5% phosphoric acid. The column temperature was 35 degrees C, the detective wavelength was set at 268 nm.
Results:
Eleven batches of Chrysanthemi Flos samples composed of both sun-dried and sulfur-fumigated were analyzed and evaluated respectively. The method of clustering analysis was used, and the chromatographic fingerprint common pattern was finally established.
Conclusion:
The HPLC fingerprint method is reliable and suitable for quality evaluation of Chrysanthemi Flos before and after sulfur-fumigation.
