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Updated: May 16, 2026
Lethality Bioassay Using Artemia salina L.
Published on: October 11, 2022
[Isolation and identification of brine shrimp lethal activities from Derris trifoliata]
Cheng Jiang1, Ben-qiang Xiong, Xi-min Qiu
1Department of Pharmacy, Medical College, Hunan Normal University, Changsha 410006, China. dangdangli@tom.com
Objective:
To study the brine shrimp lethal activities of the roots and stems from Derris trifoliata.
Methods:
The biological activity of the athyl acetate extract was tested by the 2nd-instar of Artemia franciscana by the method of Silica gel column chromatography, Sephadex LH-20 column chromatography, Semipreparative HPLC, and their structures were elucidated by spectral analysis.
Results:
Eleven compounds were isolated from the athyl acetate extract, and LD50 s of 12a-hydroxyrotenone, tephrosin, rotenone and deguelin were 0.365, 0.236, 0.060 and 0.734 microg/mL, respectively.
Conclusion:
The results of bioassay show that 12a-hydroxyrotenone, tephrosin,rotenone and deguelin have strong brine shrimp lethal activity.
