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Updated: May 16, 2026
A 3D Organotypic Melanoma Spheroid Skin Model
Published on: May 18, 2018
CD10 expression by melanoma cells is associated with aggressive behavior in vitro and predicts rapid metastatic
Marianne Thomas-Pfaab1, Jean-Philippe Annereau, Coline Munsch
1mariannethomas@ymail.com
Background:
No biological or molecular marker of primary melanoma tumor cells has been shown to predict clinical outcome in melanoma.
Objective:
To determine whether CD10, CD133, nestin and CD20 may evaluate the prognosis of melanoma.
Methods:
The differential expression of these molecules was assessed in pairs of cell lines. We evaluated, by both immunohistochemical staining and RT-qPCR, their expression in a cohort of 32 patients (68 samples) with a history of metastatic melanoma, divided into two groups according to their clinical outcome profile.
Results:
CD10 over expression in cancer cell lines was associated with more aggressive behavior in vitro. A CD10-positive staining was more frequent in patients in the "rapidly progressive" group than those in the "long survivor" group (23/35 versus 2/18, p<10(-4)). CD10 expression was associated with a lower median overall survival (1.15 year - IQR: [0.50-2.58] versus 4.27 - IQR: [1.66-6.33]; p=10(-4)). The Odds Ratio of displaying a "rapidly progressive" melanoma when tumor cells expressed CD10 was 15 (95% confidence interval: [3-78]). After adjusting for confounding factors, CD10 expression in melanoma tumor cells remained associated with an increased risk of death and more rapid disease progression (p=6×10(-4); HR=3.71).
Conclusion:
CD10 may predict clinical outcome in melanoma patients.
