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Updated: May 16, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Fast multicolor 3D imaging using aberration-corrected multifocus microscopy
Sara Abrahamsson1, Jiji Chen, Bassam Hajj
1Joint Graduate Group in Bioengineering, University of California, San Francisco/University of California, Berkeley, San Francisco, California, USA. sara.abrahamsson@gmail.com
Abstract:
Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.
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