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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...

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Related Experiment Video

Updated: May 16, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Localized thermal mapping using coherent anti-Stokes Raman spectroscopy.

Hope T Beier1, Gary D Noojin, Benjamin A Rockwell

  • 1Air Force Research Laboratory, 4141 Petroleum Road, Building 3260 Fort Sam, Houston, TX 78234, USA. hopebeier@gmail.com

Journal of Biomedical Optics
|December 11, 2012
PubMed
Summary

Coherent anti-Stokes Raman scattering (CARS) spectroscopy precisely measures micro-scale temperatures in water and saline solutions. This technique maps local temperature variations with high accuracy, offering a new tool for thermal analysis.

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Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
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Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

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Published on: April 28, 2022

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope
12:54

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Published on: July 17, 2016

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09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Area of Science:

  • Spectroscopy
  • Thermal Measurement
  • Microscopy

Background:

  • Accurate micro-scale thermal measurements are crucial in various scientific fields.
  • Existing methods may lack the spatial resolution or sensitivity required for certain applications.

Purpose of the Study:

  • To investigate Coherent Anti-Stokes Raman Scattering (CARS) spectroscopy for micro-scale thermal measurements.
  • To assess the accuracy and applicability of CARS for temperature determination in liquids.

Main Methods:

  • Utilized a femtosecond oscillator to generate a broadband Stokes pulse via a photonic crystal fiber.
  • Analyzed CARS signals from OH-stretching modes (3000-3600 cm(-1)) to correlate with temperature.
  • Employed black-dyed microspheres as localized thermal sources for mapping.

Main Results:

  • Demonstrated a temperature correlation with CARS signals in water (± 1°C accuracy).
  • Achieved ± 1.5°C accuracy for phosphate-buffered saline temperature measurements.
  • Successfully mapped local temperature variations at a microscopic level.

Conclusions:

  • CARS spectroscopy is a viable and accurate method for micro-scale temperature measurements in liquids.
  • The technique offers high precision for analyzing thermal gradients and distributions.
  • This approach provides a powerful tool for advanced thermal analysis in microfluidic and biological systems.