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Updated: May 16, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Mosaic acquisition and processing for optical-resolution photoacoustic microscopy.

Peng Shao¹, Wei Shi, Ryan K W Chee

¹University of Alberta, Department of Electrical & Computer Engineering, Edmonton T6G2V4, Canada.

Journal of Biomedical Optics

|December 11, 2012

Summary

We developed a faster hybrid optical and mechanical-scanning photo-acoustic microscopy system. This new system achieves panoramic imaging by stitching mosaic image patches, significantly reducing data acquisition time.

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Three-dimensional Optical-resolution Photoacoustic Microscopy

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Switchable Acoustic and Optical Resolution Photoacoustic Microscopy for In Vivo Small-animal Blood Vasculature Imaging

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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Area of Science:

Biomedical Optics
Microscopy
Photoacoustic Imaging

Background:

Data acquisition time in optical-resolution photoacoustic microscopy (OR-PAM) is constrained by laser pulse repetition rate (PRR) and scanning speed.
Optical-scanning OR-PAM offers speed but is limited by field of view due to ultrasound transducer sensitivity.

Purpose of the Study:

To introduce a hybrid optical and mechanical-scanning OR-PAM system.
To improve imaging speed and field of view through mosaic data acquisition and processing.

Main Methods:

Utilized a hybrid system combining fast-scanning mirrors with mechanical scanning.
Employed a 600 kHz PRR diode-pumped, 532-nm fiber laser.
Implemented mosaic data acquisition and stitching for panoramic image generation.

Main Results:

The hybrid system demonstrated significantly enhanced imaging speed.
Achieved panoramic imaging by systematically acquiring and stitching mosaic image patches.
The proposed system proved to be at least 20 times faster than previous OR-PAM systems.

Conclusions:

The hybrid optical and mechanical-scanning OR-PAM system offers a substantial speed improvement for large-area imaging.
Mosaic data acquisition and processing effectively overcome field-of-view limitations.
This advancement has the potential to accelerate applications requiring rapid, high-resolution photoacoustic imaging.