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Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses

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Advances towards a Marker-Assisted Selection Breeding Program in Prairie Cordgrass, a Biomass Crop.

K R Gedye¹, J L Gonzalez-Hernandez, V Owens

¹Department of Plant Sciences, South Dakota State University, Brookings, SD 57007, USA.

International Journal of Plant Genomics

|December 11, 2012

Summary

Prairie cordgrass breeding is advancing with new molecular tools. Researchers developed simple sequence repeat (SSR) markers, confirming prairie cordgrass is not self-incompatible and enabling marker-assisted selection for biomass crop improvement.

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Breeding by Design for Functional Rice with Genome Editing Technologies

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Area of Science:

Plant breeding
Genomics
Bioenergy crops

Background:

Prairie cordgrass (Spartina pectinata) is a North American native perennial grass with potential as a cellulosic biomass crop for biofuel production.
Limited research exists on breeding strategies for prairie cordgrass, hindering its development as a commercial crop.

Purpose of the Study:

To initiate the development of a breeding program for prairie cordgrass.
To create molecular tools for understanding its reproductive biology and facilitating breeding.

Main Methods:

Developed genomic libraries enriched for simple sequence repeat (SSR) motifs.
Sequenced SSR-enriched clones to identify SSR regions and design primers.
Amplified SSR markers using polymerase chain reaction (PCR) and validated crossing methods.

Main Results:

Identified 70 SSR regions and developed primers for 35 amplified markers.
Confirmed that prairie cordgrass is not self-incompatible, allowing crosses without emasculation.
Validated the crossing methodology for prairie cordgrass.

Conclusions:

The developed SSR markers are valuable for prairie cordgrass breeding.
This research lays the foundation for a molecular map and marker-assisted selection (MAS) in prairie cordgrass breeding programs.
Facilitates the improvement of prairie cordgrass for enhanced cellulosic biomass production.