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Association analysis between SNPs in IL-28B gene and the progress of hepatitis B infection in Han Chinese

Jie Chen1, Lanlan Wang, Yi Li

  • 1Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, PR China.

Plos One
|December 11, 2012
PubMed

Insights

The IL-28B rs12979860 C/T polymorphism T allele is more common in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) patients than in liver cirrhosis (LC) patients. This allele may increase HCC development risk.

Area of Science:

  • Genetics
  • Hepatology
  • Virology

Background:

  • Hepatitis B virus (HBV) infection can lead to liver cirrhosis (LC) and hepatocellular carcinoma (HCC).
  • Interleukin-28B (IL-28B) gene polymorphisms have been associated with HBV infection outcomes.
  • Single nucleotide polymorphisms (SNPs) in IL-28B may influence disease progression.

Purpose of the Study:

  • To investigate the association between IL-28B gene SNPs and the occurrence of LC and HCC in HBV-infected individuals.
  • To determine if specific IL-28B polymorphisms are risk factors for developing advanced liver disease.

Main Methods:

  • A candidate gene association study was conducted on Chinese Han subjects.
  • Genomic DNA was analyzed from 406 LC and 406 HCC patients caused by chronic hepatitis B (CHB).
  • SNP rs12979860 in IL-28B was detected using high-resolution melting curve analysis, with verification in independent cohorts.

Main Results:

  • A significant difference in the frequency of rs12979860 was observed between CHB patients who progressed to LC or HCC (p = 0.046).
  • Patients with HCC more frequently carried the T allele at rs12979860 compared to LC patients.
  • These findings were consistent in an independent sample verification.

Conclusions:

  • The T allele of the IL-28B rs12979860 C/T polymorphism is more prevalent in HCC patients than in LC patients.
  • Carriage of the IL-28B rs12979860 T allele may increase the risk of developing HCC.
  • IL-28B gene polymorphism might influence the symptomatic specificity and progression of hepatitis B infection.
Abstract

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